The transport of chloride ions in the leaves of Vallisneria is a plasmatic process dependent on cell metabolism. It is checked by a wound stimulus and promoted by administering fructose. Uptake from the medium is regulated by a process in the outer layer of the cytoplasm. KCN is an inhibitor of this process. There is an active secretion-process by which chloride ions from the cytoplasm are accumulated in the vacuoles. This process is inhibited by 2-4, dinitrophenol. To Mrs H Knobbe-Meester I am greatly indebted for the careful execution and help with the experiments and the analyses in the years 1947 to 1952.