Ammonium sulphate precipitation and filtration through Sephadex G-100 were used to purify the lAA-oxidase present in root nodules and roots of Lupinus luteus L. During the gel filtration all fractions showing lAA-oxidase activity also showed polyphenoloxidase activity – although this is presumably a different enzyme – and peroxidase activity, the latter being almost strictly in parallel with lAA-oxidase activity, even after ion-exchange chromatography. We conclude that the same lAA-degrading enzyme system is present in root nodules and roots.

Acta botanica neerlandica

CC BY 3.0 NL ("Naamsvermelding")

Koninklijke Nederlandse Botanische Vereniging

A.M. Mennes. (1973). The indole-3-acetic acid oxidase of Lupinus luteus L. I. A qualitative comparison of the activity of this enzyme in root nodules and roots. Acta botanica neerlandica, 22(6), 694–705.