On the relative extractability of Chlorophyl a685 and Chlorophyll b complexes by two detergents

Treatment of Phaseolus vulgaris chloroplast fragments with 8 or 4 mM digitonin as well as 8 or 4 mM sodium dodecyl sulfate results in a proportionally higher solubilization of the chlorophyll a685 complex and a lower one of chlorophyll b as compared to the extractability of the remaining chlorophyll a forms. The results are discussed with respect to previous data suggesting the occurrence of a chlorophyll /(-chlorophyll «685-protein complex. It may be that both pigment forms are bound to different polypeptides of this complex.