The use of DNA-DNA hybridization for determination of the relationship between some blue-green algae (Cyanophyceae)

A DNA-DNA hybridization membrane filter method suitable for blue-green algal DNA was developed. One of the main obstacles, the high amount of aspecific binding of the blue-green algal DNA to filters, could be resolved by means of an additional purification step involving isopycnic centrifugation of the thoroughly purified DNA. Application on DNA of several strains of blue-green algae indicates that this method is useful for taxonomic purposes.