The ultrastructure of generative cells and vegetative cells of Papaver dubium L. pollen was studied with low-temperature scanning electron microscopy (LTSEM). Liquid propane freezing resulted in a very fast fixation. The on-line fracturing and observation of the pollen grains was performed at — 130°C. The specimens were fully hydrated and did not undergo morphological changes during the procedure. Unfractured and cross fractured organelles, generative cells, vegetative nuclei and membranes are visible in three-dimensional images. The LTSEM micrographs show a lobed vegetative nucleus with many nuclear pores and a wrinkled generative cell in longitudinal direction. The vegetative nucleus and the generative cell are situated close to each other but structural connections are not observed.

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Acta botanica neerlandica

CC BY 3.0 NL ("Naamsvermelding")

Koninklijke Nederlandse Botanische Vereniging

A.C. van Aelst, T. Mueller, M. Dueggelin, & R. Guggenheim. (1989). Three-dimensional observations on freeze-fractured frozen hydrated Papaver dubium pollen with cryo-scanning electron microscopy. Acta botanica neerlandica, 38(1), 25–30.