After a pretreatment in water, aperturate pollen grains can be led to formation of “Instant Pollen Tubes” (IPoT) by treatment with strong inorganic acids, such as sulfuric acid. This IPoT formation is a pseudogermination dependent on the living state of pollen and a distinct period of swelling and enzyme activation. Conditions of pretreatment and acid treatment are investigated. IPoT formation seems to be a suitable object for further investigation of the molecular structure of the inline and the dependence of pollen tube formation on initiation of enzyme activation.