Induction of embryoids in reproductive and vegetative tissues of Ranunculus sceleratus L. in vitro

Isolated sepals, petals and anthers grown on a basal medium supplemented with coconut milk and 2,4-D yielded callus and embryoids resulting in plantlets. Carpels, however, developed callus and embryoids on the basal medium alone. Seeds germinated in vitro and produced seedlings but failed to produce embryoids on their stem, in contrast to plantlets raised from embryoids. Formation of callus and embryoid differentiation was also noticed in cultures of parts of seedlings, shoot tips, segments of root, stem, petiole and lamina of in vivo and in vitro plants.