Studies on phloem exudation from Yucca flaccida Haw. XIII. Evidence for the occurrence of a cytokinin nucleotide in the exudate
Acta botanica neerlandica , Volume 23 - Issue 4 p. 541- 548
Phloem exudate of Yucca was analysed for cytokinin activity. Aftei partition in a water- and butanol-1 fraction, almost no cytokinin activity was found in the butanol phase (cytokinin base and nucleosides). Application of alkaline phosphatase to the water layer gave rise to a strong positive cytokinin response with the soy-bean callus test, an amount of approximately 520 ;j.g zeatin equivalent per litre being found in the exudate after treatment with alkaline phosphatase. The results indicate that a cytokinin, presumably zeatin, is translocated as a nucleotide.
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C.R. Vonk. (1974). Studies on phloem exudation from Yucca flaccida Haw. XIII. Evidence for the occurrence of a cytokinin nucleotide in the exudate. Acta botanica neerlandica, 23(4), 541–548.
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