1977
Light and electron microscopic autoradiography of cell-wall regeneration by Schizophyllum commune protoplasts
Publication
Publication
Acta botanica neerlandica , Volume 26 - Issue 1 p. 43- 52
Regenerating protoplasts of the basidiomycete Schizophyllum commune were labelled with radioactive N-acetylglucosamine. The label was mainly incorporated in the alkali-insoluble wall fractions R-glucan (containing some hexosamine) and chitin. Light microscopic autoradiography showed a uniformly dispersed incorporation of label in primary cells and chaincells but emerged hyphae revealed a highly localized incorporation at the hyphal apex. Electron microscopic autoradiography showed that the label mainly appeared just outside the plasmalemma in the wall suggesting that chitin and R-glucan are synthesized at the plasmalemma/cell wall interface.
Additional Metadata | |
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Acta botanica neerlandica | |
CC BY 3.0 NL ("Naamsvermelding") | |
Organisation | Koninklijke Nederlandse Botanische Vereniging |
P. van der Valk, & J.G.H. Wessels. (1977). Light and electron microscopic autoradiography of cell-wall regeneration by Schizophyllum commune protoplasts. Acta botanica neerlandica, 26(1), 43–52. |