The two pigment systems, with optimum activity at 675 nm and 750 nm, that are responsible for light dependent oxygen uptake by Anacystis nidulans remain active in the presence of DCMU. The activity of the 750 nm system is increased at the beginning of the phase of exponential growth when L-glutamate has been added to the growth medium. We presume that variations in growth conditions may induce the formation of endogenous reductants that cause increased oxygen uptake. Experiments with added reductants showed that Lascorbate, DCPIPH2 and TMPD are readily photooxidized in the intact cell by the 750 nm system. Abbreviations: DCMU: 3-(3,4-Dichlorophenyl)-l,1-dimethyl urea. DCPIP: 2,6-Dichlorophenol-indophenol. EDTA: Ethylene diaminetetra-acetic acid. TMPD; N,N,N’,N’-Tetramethyl-p-phenylenediamine.

Acta botanica neerlandica

CC BY 3.0 NL ("Naamsvermelding")

Koninklijke Nederlandse Botanische Vereniging

J.W. Kleinen Hammans, T. Teerlink, & J.Th. van den Berg. (1977). Light dependent oxygen uptake by Anacystis nidulans, studies with endogenous and added reductants. Acta botanica neerlandica, 26(4), 285–288.