Electron microscopic autoradiography of β-glucans synthesized by a membrane fraction from pollen tubes of Petunia hybrida
Acta botanica neerlandica , Volume 30 - Issue 1/2 p. 1- 7
Alkaline extraction of a membrane fraction from Petunia pollen tubes left an alkali-insoluble fraction that consisted of microfibrils embedded in amorphous material. The amorphous material was partially separated from the microfibrils by disaggregation with ultrasound or by solubilization with hydrogen peroxide/glacial acetic acid. Previously it was shown that incubation of the membrane fraction with UDP-[U-14C]glucose gave an alkali-insoluble fraction containing labelled fi-glucans. EM-autoradiography of this fraction, after labelling with UDP-[6-3H]glucose, showed that in vitro synthesized (3-glucans did not self-assemble but bound strongly to pre-existing microfibrils. Applicability of EM-autoradiography for confirmation of in vitro synthesis of microfibrils is discussed.
|Acta botanica neerlandica|
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J.P.F.G. Helsper. (1981). Electron microscopic autoradiography of β-glucans synthesized by a membrane fraction from pollen tubes of Petunia hybrida. Acta botanica neerlandica, 30(1/2), 1–7.
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