Using immunofluorescence techniques, microtubules were studied in differentiating cells of Raphanus sativus. Within one cell large differences in microtubule patterns occur. The results indicate a relation between cell expansion and microtubule orientation; at elongating surfaces microtubules are found perpendicular to the direction of expansion at the cells surface. At non-expanding cell surfaces, randomly oriented microtubules are found. Possibly a relation exists between the rate of elongation and the density of the microtubules.