The effect of elevated temperatures on protein synthesis in pollen from Petunia hybrida and Lilium longiflorum was investigated and compared with their effect in root-tissue tissue of Petunia. The results demonstrate that the incorporation of [35-S] methionine into protein does not change in pollen incubated at temperatures up to 40 °C, but decreased with 65% at 45 °C as compared to incorporation at 27 °C. The qualitative changes that occurred for the proteins synthesized at 27° and 40 °C were not identical for pollen from Petunia and Lilium. The proteins synthesized at 21° and 40 °C were very similar within one species. None of the changes in proteins synthesized at 40°C in pollen showed any correlation with heat shock proteins formed in rootlets from Petunia.