The patch-clamp technique is a relatively new and powerful electrophysiological technique. Its resolving power is such that ion currents can be recorded through only one protein or ion-channel. The application of this technique in higher plant physiology, its principles and advantages are discussed in this paper. Furthermore, some actual results of single-channel recordings, whole cell recordings and ATPase currents are shown and examples are given of ion-channel conductance and selectivity calculations.

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Acta botanica neerlandica

CC BY 3.0 NL ("Naamsvermelding")

Koninklijke Nederlandse Botanische Vereniging

F.J.M. Maathuis, & H.B.A. Prins. (1991). Patch-clamp studies in cell membranes of higher plants. Acta botanica neerlandica, 40(3), 197–209.