Pollen-tube subprotoplasts of Nicotiana tabacum L. regenerate a new cell-wall with a random texture and a relatively high content of P 1-3 glucan (callose). The subprotoplasts become vacuolized during culture, and both the cytoplasm and wall deposition are limited to a single site of the subprotoplast. After 8 h of culturing, wall formation becomes restricted to a very small area and a fibre-like structure evolves. Fibre formation seems to result from a spontaneous clustering of multiple wall-synthesizing sites. These sites probably give rise to the different strands seen in the fibre. Wall deposition and fibre formation take place independent of the presence of a nucleus or microtubules and actin filaments.

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Acta botanica neerlandica

CC BY 3.0 NL ("Naamsvermelding")

Koninklijke Nederlandse Botanische Vereniging

T.L.M. Rutten, M. Kroh, & B. Knuiman. (1991). Cell-wall regeneration in pollen-tube subprotoplasts. Acta botanica neerlandica, 40(3), 211–216.