Apple and sugar-beet pectins were extracted with buffers in the absence or presence of EDTA or CDTA as chelators. These pectins were highly methylated and, in the case of sugar-beet, highly acetylated. Neither the extracted pectins nor the alcohol insoluble materials from either species were recognized by a monoclonal antibody directed to calciuminduced pectin dimers (‘egg-boxes’), unless chemically de-esterified. It is therefore unlikely that such dimers exist to any large extent in vivo.

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Acta botanica neerlandica

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Koninklijke Nederlandse Botanische Vereniging

C.M.G.C. Renard, J.-F. Thibault, F. Liners, & P. van Cutsem. (1993). Immunological probing of pectins isolated or in situ. Acta botanica neerlandica, 42(2), 199–204.