In the springtime of 2011, 507 adult spiders (plus 7 identifiable subadults) representing a total of 135 species were collected in Gargano, Apulia, Italy. A list of collected spiders is given. Zelotes rinske spec. nov. is described after a single female. Dictyna innocens O. P.-Cambridge, 1872, Leptodrassus femineus (Simon, 1873), Nomisia recepta (Pavesi, 1880), Zelotes criniger Denis, 1937, Pellenes brevis (Simon, 1868), and Salticus propinquus Lucas, 1846 are reported for the first time from mainland Italy. Illustrations are provided of the problematic species Dictyna innocens O. P.-Cambridge, 1872. The poorly-known female of Araeoncus altissimus Simon, 1884 is described and depicted. 141 specimens representing 105 species were DNA barcoded.